6I9Q

Structure of the mouse CD98 heavy chain ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-22
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9184
Spacegroup name	I 4 2 2
Unit cell lengths	97.384, 97.384, 218.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.450 - 2.100
R-factor	0.1771
Rwork	0.175
R-free	0.21840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dh2
RMSD bond length	0.013
RMSD bond angle	1.583
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.450	34.450	2.200
High resolution limit [Å]	2.100	10.000	2.100
Rmerge	0.130	0.036	1.453
Rmeas	0.133	0.037	1.480
Number of reflections	31196	343	3984
<I/σ(I)>	21.43	69.04	2.82
Completeness [%]	99.9	96.1	99.9
Redundancy	26.556	22.548	28.081
CC(1/2)	1.000	1.000	0.811

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	24 % (w/v) PEG 3350, 0.1 M Bis-tris propane/HCl, 0.2 M sodium sulfate