6I9P

Iron-free state of Rana catesbeiana H' ferritin variant H54N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-08
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8856
Spacegroup name	F 4 3 2
Unit cell lengths	183.910, 183.910, 183.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.100 - 1.250
R-factor	0.11771
Rwork	0.117
R-free	0.13327
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lqh
RMSD bond length	0.009
RMSD bond angle	1.477
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.240	1.320
High resolution limit [Å]	1.250	1.250
Rmerge	0.074	0.418
Rmeas	0.079	0.450
Rpim	0.021	0.122
Number of reflections	73567	10565
<I/σ(I)>	19.1	6.2
Completeness [%]	100.0	100
Redundancy	14.1	13.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	281	1.6-2.0 M MgCl2 and 0.1 M bicine pH 9.0