6I8R

Crystal structure of an ancient sequence-reconstructed Elongation factor Tu (node 170)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97242
Spacegroup name	P 21 21 21
Unit cell lengths	57.225, 73.956, 115.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.420 - 2.000
R-factor	0.1949
Rwork	0.192
R-free	0.24170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dg1
RMSD bond length	0.016
RMSD bond angle	1.983
Data reduction software	XDS
Data scaling software	Aimless (0.5.23)
Phasing software	Auto-Rickshaw
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.420	45.420	2.050
High resolution limit [Å]	2.000	8.930	2.000
Rmerge	0.052	0.031	1.596
Rmeas	0.055	0.033	1.686
Rpim	0.018	0.012	0.534
Total number of observations	325593
Number of reflections	33564	447	2391
<I/σ(I)>	17.5
Completeness [%]	99.0	98	96.5
Redundancy	9.7	8	9.6
CC(1/2)	0.999	0.999	0.724

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		296	0.2 M Potassium Chloride, 20% w/v Polyethylene glycol 3350