6I8C
Crystal structure of the murine beta-2-microglobulin.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-03-10 |
| Detector | MAR scanner 180 mm plate |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.242, 47.603, 64.937 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.850 - 1.920 |
| R-factor | 0.18528 |
| Rwork | 0.183 |
| R-free | 0.22186 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bii |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.152 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 1.950 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Number of reflections | 8882 | 572 |
| <I/σ(I)> | 20.1 | |
| Completeness [%] | 98.6 | 96.8 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 40% PEG 6000, 100mM Tris |
