6I8A

The crystal structure of the Pol2 catalytic domain of DNA polymerase epsilon carrying a P301R substitution.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-04
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9792
Spacegroup name	P 1 2 1
Unit cell lengths	154.466, 70.257, 159.335
Unit cell angles	90.00, 112.85, 90.00

Refinement procedure

Resolution	19.957 - 2.652
R-factor	0.2315
Rwork	0.229
R-free	0.27920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m8o
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.957	19.957	2.810
High resolution limit [Å]	2.650	7.860	2.650
Rmerge	0.075	0.020	1.203
Rmeas	0.089	0.024	1.425
Number of reflections	91152	3681	14007
<I/σ(I)>	10.23	35.53	0.89
Completeness [%]	98.7	98.4	94.5
Redundancy	3.422	3.3	3.454
CC(1/2)	0.999	0.999	0.569

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	292	50mM MES pH 6.5, 150mM NaAc, 8%PEG20K