6I5M

Gamma subunit of the translation initiation factor 2 from Sulfolobus solfataricus in complex with GDP and formate ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-03
Detector	Bruker Platinum 135
Wavelength(s)	1.54179
Spacegroup name	H 3 2
Unit cell lengths	142.143, 142.143, 218.587
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.243 - 2.400
R-factor	0.1754
Rwork	0.173
R-free	0.22420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m4s
RMSD bond length	0.008
RMSD bond angle	0.986
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.243	2.500
High resolution limit [Å]	2.400	2.400
Number of reflections	33014	3784
<I/σ(I)>	10.28	2.16
Completeness [%]	98.7	99.2
Redundancy	2.91	2.81

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	2,85mM GTP, 1,33M sodium formiate, 37mM sodium cacodylate pH 6.5, 13 mM TrisHCl pH 7.5, 130 mM NaCl, 1,3 mM MgCl2