6I59
Long wavelength native-SAD phasing of Sen1 helicase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-12 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 2.7 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 91.830, 171.600, 69.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.551 - 2.950 |
R-factor | 0.1714 |
Rwork | 0.169 |
R-free | 0.21270 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.658 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXCD |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.030 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmeas | 0.032 | 0.428 |
Number of reflections | 23557 | 1653 |
<I/σ(I)> | 31.08 | 1.66 |
Completeness [%] | 99.1 | 95.2 |
Redundancy | 49.12 | 37.88 |
CC(1/2) | 0.999 | 0.693 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 277 | 18% PEG 2K 0.1M MOPS 7.2 |