6I4X

Crystal structure of SOCS2:Elongin C:Elongin B in complex with erythropoietin receptor peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	I 1 2 1
Unit cell lengths	41.287, 56.327, 203.392
Unit cell angles	90.00, 91.53, 90.00

Refinement procedure

Resolution	29.360 - 2.690
R-factor	0.1986
Rwork	0.196
R-free	0.23510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.451
Data scaling software	AutoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.360	2.820
High resolution limit [Å]	2.690	2.690
Rmerge	0.108	0.514
Number of reflections	12273
<I/σ(I)>	9.6
Completeness [%]	93.2
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	Sodium cacodylate, ethanol, HEPES, magnesium chloride