6I4M

Crystal Structure of Plasmodium berghei actin II in the Mg-ADP state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-09-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.031
Spacegroup name	P 1 21 1
Unit cell lengths	61.410, 61.510, 64.940
Unit cell angles	90.00, 92.55, 90.00

Refinement procedure

Resolution	64.880 - 1.873
R-factor	0.1446
Rwork	0.143
R-free	0.17880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cbx
RMSD bond length	0.011
RMSD bond angle	1.080
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.880	1.940
High resolution limit [Å]	1.873	1.873
Rmerge	0.038	0.295
Rmeas	0.051	0.403
Rpim	0.034	0.273
Number of reflections	73248	4975
<I/σ(I)>	15.1
Completeness [%]	93.8	63.77
Redundancy	1.9	1.7
CC(1/2)	0.999	0.826

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.3	298	23% PEG3350, 0.1M BIS-TRIS pH 6.3, 0.2M K-SCN; 20% PEG400 used for cryoprotection