6I4E
Crystal Structure of Plasmodium falciparum actin I in the Mg-ADP state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.954 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 68.520, 71.270, 109.980 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.390 - 1.220 |
R-factor | 0.1367 |
Rwork | 0.136 |
R-free | 0.15670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4cbu |
RMSD bond length | 0.011 |
RMSD bond angle | 1.167 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.390 | 1.264 |
High resolution limit [Å] | 1.220 | 1.220 |
Rmerge | 0.047 | 1.129 |
Rmeas | 0.052 | 1.250 |
Rpim | 0.022 | 0.529 |
Number of reflections | 159860 | 15809 |
<I/σ(I)> | 16.29 | |
Completeness [%] | 99.8 | 99.75 |
Redundancy | 5.4 | 5.4 |
CC(1/2) | 0.999 | 0.513 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 298 | 23% PEG3350, 0.1M BIS-TRIS pH 6.2, 0.2M K-SCN; 20% PEG400 used for cryoprotection |