6I41
Co-crystal structure of human SPOP MATH domain (wild-type) and human BRD3 fragment
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-24 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.8731 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 61.508, 55.180, 42.606 |
| Unit cell angles | 90.00, 100.39, 90.00 |
Refinement procedure
| Resolution | 19.632 - 1.900 |
| R-factor | 0.1902 |
| Rwork | 0.188 |
| R-free | 0.23850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ivv |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.772 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.800 | 1.820 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmeas | 0.058 | |
| Number of reflections | 13067 | 534 |
| <I/σ(I)> | 9.8 | |
| Completeness [%] | 98.3 | |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 100 mM Tris pH 8.5, 200 mM NaCl, 25% (w/v) PEG3350 |
