6I3V

x-ray structure of the human mitochondrial PRELID1 in complex with TRIAP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-31
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.92
Spacegroup name	P 63 2 2
Unit cell lengths	127.312, 127.312, 177.390
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	93.642 - 1.980
R-factor	0.2072
Rwork	0.205
R-free	0.24520
RMSD bond length	0.016
RMSD bond angle	1.298
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.642	2.050
High resolution limit [Å]	1.980	1.980
Number of reflections	59554
<I/σ(I)>	19
Completeness [%]	99.0
Redundancy	19.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM CHES/ Sodium hydroxide pH 9.5 200 mM Sodium chloride 40% (v/v) PEG 300