6I3O

Crystal structure of DEAH-box ATPase Prp22

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9919
Spacegroup name	P 21 21 21
Unit cell lengths	67.530, 93.640, 218.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	86.060 - 3.250
R-factor	0.2483
Rwork	0.247
R-free	0.26370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fa5
RMSD bond length	0.012
RMSD bond angle	1.723
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	86.060	86.060	3.350
High resolution limit [Å]	3.250	50.000	3.250
Rmerge	0.113	0.016	1.060
Rmeas	0.126	0.023	1.181
Total number of observations	116904
Number of reflections	22460	6	1859
<I/σ(I)>	12.04	23.84	1.52
Completeness [%]	99.5	66.7	96.3
Redundancy	5.205	1.333	4.976
CC(1/2)	0.998		0.657

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	100mM Bis-Tris propane pH6.5, 400mM K-Thiocyanate, 20% PEG3350