6I3J
Bilirubin oxidase from Myrothecium verrucaria in complex with ferricyanide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 134.382, 203.846, 226.744 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.300 - 2.590 |
| R-factor | 0.1618 |
| Rwork | 0.160 |
| R-free | 0.22570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xll |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.350 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.300 | 2.670 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.144 | 0.858 |
| Rmeas | 0.157 | 0.963 |
| Rpim | 0.061 | 0.428 |
| Number of reflections | 48305 | 4411 |
| <I/σ(I)> | 13.5 | 2 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 6.5 | 4.9 |
| CC(1/2) | 0.994 | 0.608 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.1 | 291.15 | 14% (w/v) PEG 3350, 0.1 M succinic acid, protein concentration 25 mg/ml |
