6I37
Crystal structure of nv1Pizza6-AYW, a circularly permuted designer protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-18 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00003 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.256, 55.977, 69.352 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.559 - 1.540 |
| R-factor | 0.1912 |
| Rwork | 0.190 |
| R-free | 0.22390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6f0q |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.872 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.559 | 1.570 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.056 | 0.138 |
| Rmeas | 0.066 | 0.162 |
| Rpim | 0.035 | 0.085 |
| Number of reflections | 27927 | 1288 |
| <I/σ(I)> | 17.6 | 7.9 |
| Completeness [%] | 98.3 | 93.6 |
| Redundancy | 6.3 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 1.6M Ammonium sulfate + 25% Glycerol |
