6I35
Crystal structure of human glycine decarboxylase (P-protein) bound with pyridoxyl-glycine-5'-monophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-14 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.660, 123.440, 197.670 |
| Unit cell angles | 90.00, 97.93, 90.00 |
Refinement procedure
| Resolution | 49.330 - 2.000 |
| R-factor | 0.21627 |
| Rwork | 0.215 |
| R-free | 0.24047 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6i33 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.341 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.330 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmeas | 0.170 | |
| Number of reflections | 271538 | 36741 |
| <I/σ(I)> | 5.3 | 1.1 |
| Completeness [%] | 99.0 | 98.8 |
| Redundancy | 4.6 | 4.5 |
| CC(1/2) | 0.995 | 0.484 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M HEPES pH 7, 6 % PEG3350, 10 mM DTT |
