6I2Q

Crystal structure of the wild-type SucA domain of Mycobacterium smegmatis KGD (alpha-ketoglutarate decarboxylase), in complex with GarA

Replaces:  2XT9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9800
Spacegroup name	C 2 2 21
Unit cell lengths	141.250, 167.846, 102.808
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.480 - 2.150
R-factor	0.165
Rwork	0.163
R-free	0.19100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yic
RMSD bond length	0.010
RMSD bond angle	0.990
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.830	2.200
High resolution limit [Å]	2.150	2.150
Rmerge	0.062	0.747
Rpim	0.030	0.361
Number of reflections	65695
<I/σ(I)>	16.6
Completeness [%]	98.9
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	100 mM Hepes-Na pH 7.0, 50% MPD