6I2M

Crystal structure of vaccinia virus protein A55 BTB-Back domain in complex with human Cullin-3 N-terminus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	198.820, 42.520, 148.770
Unit cell angles	90.00, 128.41, 90.00

Refinement procedure

Resolution	58.290 - 2.300
R-factor	0.267
Rwork	0.266
R-free	0.28200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4eoz 1r29
Data reduction software	DIALS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.230	2.360
High resolution limit [Å]	2.300	2.300
Rmerge	0.140	17.224
Rmeas	0.146	17.953
Rpim	0.040	4.996
Number of reflections	44144	3279
<I/σ(I)>	8.1	0.1
Completeness [%]	100.0	99.9
Redundancy	12.9	12.6
CC(1/2)	0.999	0.302

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	3.29% tacsimate pH 6.5, 9.92% PEG3350