6I1D
Structure of the Ysh1-Mpe1 nuclease complex from S.cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9159 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.380, 124.270, 63.450 |
| Unit cell angles | 90.00, 103.21, 90.00 |
Refinement procedure
| Resolution | 62.135 - 2.280 |
| R-factor | 0.175 |
| Rwork | 0.173 |
| R-free | 0.22190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i7v 2c7h |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.545 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.130 | 2.320 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.065 | 1.130 |
| Rmeas | 0.077 | 1.346 |
| Rpim | 0.041 | 0.716 |
| Number of reflections | 29524 | 1442 |
| <I/σ(I)> | 12.3 | 1.3 |
| Completeness [%] | 99.1 | 99.4 |
| Redundancy | 3.39 | 3.4 |
| CC(1/2) | 0.999 | 0.578 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.7 | 293 | 80 ul reservoir of 26 % w/v PEG 3000, 0.1 M CHES pH 8.7 The final drop of 400 nl comprised 200 nl protein and 200 nl crystallization buffer |
