6I0Q

Crystal structure of BTB domain of KCTD16 hexamer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-03
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.8
Spacegroup name	P 65
Unit cell lengths	62.407, 62.407, 41.861
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.095 - 2.300
R-factor	0.1894
Rwork	0.186
R-free	0.23460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5a15
RMSD bond length	0.003
RMSD bond angle	0.564
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11_2567: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.860	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.200	1.372
Rmeas	0.229	1.540
Rpim	0.104	0.690
Number of reflections	4228	403
<I/σ(I)>	8.1	1.8
Completeness [%]	100.0	100
Redundancy	9.1	9.4
CC(1/2)	0.994	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M ammonium sulfate and 20% PEG 3350