6I0D
Respiratory complex I from Thermus thermophilus with bound Decyl-Ubiquinone
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.900 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 94.700, 328.000, 260.900 |
Unit cell angles | 90.00, 100.40, 90.00 |
Refinement procedure
Resolution | 29.867 - 3.600 |
R-factor | 0.2197 |
Rwork | 0.214 |
R-free | 0.22470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hea |
RMSD bond length | 0.005 |
RMSD bond angle | 0.816 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.000 | 3.790 |
High resolution limit [Å] | 3.600 | 3.600 |
Rmerge | 0.262 | 1.605 |
Rpim | 0.243 | 1.523 |
Number of reflections | 173452 | 25274 |
<I/σ(I)> | 4 | 0.6 |
Completeness [%] | 96.3 | 95.8 |
Redundancy | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 296 | 100 mM Bis-Tris pH 6.0, 23% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201 |