6HZG
BP0997, GH138 enzyme targeting pectin rhamnogalacturonan II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9787 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 55.298, 68.379, 220.158 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.100 - 1.600 |
| R-factor | 0.156 |
| Rwork | 0.154 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.618 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.390 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 100505 | |
| <I/σ(I)> | 5.9 | 1.7 |
| Completeness [%] | 90.5 | 84.7 |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 16 % PEG 8000, 100 mM Tris pH 8.4, 9% Butanol |
