6HZ3
THE GLIC PENTAMERIC LIGAND-GATED ION CHANNEL MUTANT E243G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2015-07-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.450, 129.070, 159.610 |
| Unit cell angles | 90.00, 101.03, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.150 |
| R-factor | 0.2313 |
| Rwork | 0.230 |
| R-free | 0.24890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.120 | 3.230 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Number of reflections | 61001 | |
| <I/σ(I)> | 5.6 | 2 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4 | 293 | PH 4, VAPOR DIFFUSION, TEMPERATURE 293K,12-14.5% PEG4K; 15% GLycerol; 400 mM NaSCN; 3% DMSO; 100mM NaAcetate pH 4 |
