6HYY
Human phosphoserine phosphatase with serine and phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-04-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97857 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 49.140, 129.140, 155.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.947 - 1.566 |
| R-factor | 0.1961 |
| Rwork | 0.195 |
| R-free | 0.22560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nnl |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.947 | 1.622 |
| High resolution limit [Å] | 1.566 | 1.566 |
| Rmerge | 0.014 | 0.244 |
| Rmeas | 0.020 | 0.345 |
| Rpim | 0.014 | 0.244 |
| Number of reflections | 69622 | 6498 |
| <I/σ(I)> | 22.3 | |
| Completeness [%] | 99.3 | 93.82 |
| Redundancy | 2 | 2 |
| CC(1/2) | 1.000 | 0.897 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.05 M CaCl2; sodium cacodylate 0.1 M pH 6.5; PEG 2000 20% |
