6HYS
Crystal structure of DHX8 helicase domain bound to ADP at 2.6 angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92044 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.960, 167.940, 137.110 |
| Unit cell angles | 90.00, 92.71, 90.00 |
Refinement procedure
| Resolution | 37.590 - 2.600 |
| R-factor | 0.183 |
| Rwork | 0.182 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2XAU and 3I4U |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.140 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.490 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.120 | 0.818 |
| Number of reflections | 92707 | |
| <I/σ(I)> | 5.8 | 1.3 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.6 | 3.7 |
| CC(1/2) | 0.992 | 0.319 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 15% 2-Methyl-2,4-pentanediol, 100mM Sodium Acetate, 6% DMSO |
