6HYI
Regulatory subunit of a cAMP-independent protein kinase A from Trypanosoma cruzi at 1.4 A resolution in complex with inosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-20 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.979179 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.740, 88.529, 46.020 |
| Unit cell angles | 90.00, 98.28, 90.00 |
Refinement procedure
| Resolution | 45.540 - 1.399 |
| R-factor | 0.181488713943 |
| Rwork | 0.180 |
| R-free | 0.20110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6flo |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.880 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.529 | 1.407 |
| High resolution limit [Å] | 1.399 | 1.399 |
| Rmeas | 0.047 | 0.643 |
| Number of reflections | 124652 | |
| <I/σ(I)> | 10.01 | |
| Completeness [%] | 99.3 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 20% PEG 3350, 0.2M Magnesium acetate |
