6HYH
Crystal structure of MSMEG_1712 from Mycobacterium smegmatis in complex with Beta-D-Fucofuranose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 98 |
Detector technology | PIXEL |
Collection date | 2018-10-13 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 118.065, 118.065, 232.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.620 - 2.500 |
R-factor | 0.2123 |
Rwork | 0.211 |
R-free | 0.23790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6hbd |
RMSD bond length | 0.010 |
RMSD bond angle | 1.389 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.485 | 2.806 |
High resolution limit [Å] | 2.500 | 2.512 |
Number of reflections | 28372 | |
<I/σ(I)> | 10.4 | |
Completeness [%] | 99.9 | |
Redundancy | 27.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | sodium chloride, magnesium chloride, Tris pH 7.5, PEG 1500 |