6HXB
SERCA2a from pig heart
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2014-09-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 116.995, 51.776, 125.604 |
| Unit cell angles | 90.00, 106.13, 90.00 |
Refinement procedure
| Resolution | 41.724 - 4.000 |
| Rwork | 0.310 |
| R-free | 0.35440 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.533 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.700 | 4.500 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Number of reflections | 12416 | |
| <I/σ(I)> | 4.2 | |
| Completeness [%] | 98.7 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292.15 | 21% PEG2000 monomethyl ether, 20% glycerol, 100 mM NaCl, 5% tert-BuOH and 2.4% Zwittergent-3-08 |
