6HW3
Yeast 20S proteasome in complex with 13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-30 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 135.510, 299.280, 145.420 |
| Unit cell angles | 90.00, 112.95, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.600 |
| R-factor | 0.19239 |
| Rwork | 0.191 |
| R-free | 0.21486 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cz4 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.103 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.066 | |
| Number of reflections | 321242 | |
| <I/σ(I)> | 12.3 | 2.7 |
| Completeness [%] | 98.5 | 99.2 |
| Redundancy | 3.1 | 0.572 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 20 mM MgAC2, 13% MPD, 0.1 M MES |
