6HVD

Human SLK bound to a maleimide inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 41 2 2
Unit cell lengths	69.871, 69.871, 164.301
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.298 - 1.630
R-factor	0.1874
Rwork	0.186
R-free	0.20750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jfm
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER (2.7.17)
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.298	1.670
High resolution limit [Å]	1.630	1.630
Rmerge	0.055
Rmeas	0.057
Rpim	0.016	0.756
Number of reflections	51803	3773
<I/σ(I)>	20
Completeness [%]	100.0	100
Redundancy	12.8	12.9
CC(1/2)	1.000	0.589

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.20M Na2SO4; 0.1M BTProp pH 8.5; 20.0% PEG 3350; 10.0% EtGly