6HV4
Yeast 20S proteasome with human beta2i (1-53) in complex with ONX 0914
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-10 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 134.990, 298.780, 143.440 |
Unit cell angles | 90.00, 112.52, 90.00 |
Refinement procedure
Resolution | 15.000 - 3.000 |
R-factor | 0.1853 |
Rwork | 0.184 |
R-free | 0.21382 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5cz4 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.225 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.100 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.093 | 0.573 |
Number of reflections | 203267 | |
<I/σ(I)> | 10 | 3.2 |
Completeness [%] | 97.2 | 99 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 20 mM MgAC2, 13% MPD, 0.1 M MES |