6HUE
ParkinS65N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-17 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.431, 66.870, 206.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.980 - 2.850 |
| R-factor | 0.22449 |
| Rwork | 0.223 |
| R-free | 0.25486 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5c1z |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.579 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.980 | 2.950 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.125 | |
| Number of reflections | 21291 | |
| <I/σ(I)> | 4.5 | |
| Completeness [%] | 96.1 | |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 100mM BIS-TRIS pH 5.5, 200mM LiSO4 and 20% PEG3350 |
