6HUA
the competence regulator ComR from Streptococcus vestibularis in complex with its cognate signaling peptide XIP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.978570 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.190, 84.030, 152.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.873 - 3.389 |
| R-factor | 0.2477 |
| Rwork | 0.245 |
| R-free | 0.30100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jub |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.493 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.590 |
| High resolution limit [Å] | 3.389 | 3.390 |
| Rmeas | 0.349 | |
| Number of reflections | 11266 | 1748 |
| <I/σ(I)> | 15.16 | 1.51 |
| Completeness [%] | 99.7 | 98.5 |
| Redundancy | 70.99 | 70.87 |
| CC(1/2) | 0.999 | 0.720 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 290 | 12% PEG 4000, 0.1 M MgCl2, 0.1 M NaCl, 0.1 M tri-sodium citrate, 10 mg/ml protein concentration |
