6HTW
Crystal structure of an ancient sequence-reconstructed Elongation factor Tu (node 184)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 82.203, 106.122, 112.472 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 112.470 - 1.800 |
R-factor | 0.199 |
Rwork | 0.197 |
R-free | 0.23730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1efc |
RMSD bond length | 0.020 |
RMSD bond angle | 2.037 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.23) |
Phasing software | Auto-Rickshaw |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 112.470 | 49.690 | 1.830 |
High resolution limit [Å] | 1.800 | 9.850 | 1.800 |
Rmerge | 0.055 | 0.027 | 1.819 |
Rmeas | 0.060 | 0.030 | 1.993 |
Rpim | 0.025 | 0.012 | 0.805 |
Number of reflections | 91686 | 656 | 4407 |
<I/σ(I)> | 13.9 | ||
Completeness [%] | 99.8 | 98.9 | 97.8 |
Redundancy | 5.9 | 5.5 | 6 |
CC(1/2) | 0.999 | 0.999 | 0.425 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 296 | 10% PEG 8000, 20% ethylene glycol, 20 mM each of 1,6-hexanediol, 1-butanol 1-2-propanediol 2-propanol 1,4-butaediol 1,3-propanediol and 0.1 mM MES/imidazole, pH6.5 |