6HTL

Measles Phosphoprotein Coiled-Coil Domain IPKI Variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979110
Spacegroup name	I 4 2 2
Unit cell lengths	33.450, 33.450, 275.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.940 - 2.300
R-factor	0.254
Rwork	0.252
R-free	0.28500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zd0
RMSD bond length	0.010
RMSD bond angle	1.130
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.940	2.260
High resolution limit [Å]	2.200	2.200
Rmerge	0.070	2.110
Rmeas	0.072	2.202
Rpim	0.016	0.592
Number of reflections	4440	287
<I/σ(I)>	15.8	1
Completeness [%]	99.2	92.1
Redundancy	22.3	13.8
CC(1/2)	0.999	0.956

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		300	PEG 4000, Calcium Chloride, Trizma