6HTJ
Crystal structure of the translation recovery factor Trf from Sulfolobus solfataricus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-14 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.00004 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.291, 58.493, 101.366 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.304 - 1.650 |
R-factor | 0.1923 |
Rwork | 0.192 |
R-free | 0.20710 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.177 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXDE |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.304 | 1.709 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.145 | 1.238 |
Rmeas | 0.150 | 1.292 |
Rpim | 0.041 | 0.363 |
Number of reflections | 29528 | 2817 |
<I/σ(I)> | 13.32 | |
Completeness [%] | 99.8 | 98.26 |
Redundancy | 13.5 | 12.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 6.5 | 277.5 | 50 mM Bis Tris pH 6.5, 50 mM NaCl, 5 mM beta-mercapthoethanol |