6HSP
Crystal structure of the zebrafish peroxisomal SCP2-thiolase (type-1) in complex with CoA and octanoyl-CoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.2822 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.180, 81.320, 96.630 |
| Unit cell angles | 90.00, 126.08, 90.00 |
Refinement procedure
| Resolution | 42.880 - 1.730 |
| R-factor | 0.19192 |
| Rwork | 0.190 |
| R-free | 0.22733 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6hrv |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.558 |
| Data reduction software | XDS (November 3, 2014) |
| Data scaling software | XDS (November 3, 2014) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.880 | 1.830 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rpim | 0.060 | 0.380 |
| Number of reflections | 85694 | 12971 |
| <I/σ(I)> | 8.42 | |
| Completeness [%] | 99.1 | 97.6 |
| Redundancy | 3.2 | 2.6 |
| CC(1/2) | 0.996 | 0.403 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 100 mM MOPS, pH 7.0, 10 % PEG6000, 20% glycerol |
