6HS5
N-terminal domain including the conserved ImpA_N region of the TssA component of the type VI secretion system from Burkholderia cenocepacia
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-07-24 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.04434 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 49.670, 125.740, 45.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.210 - 1.800 |
R-factor | 0.17966 |
Rwork | 0.177 |
R-free | 0.22594 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.567 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | SHELXCD |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.670 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.043 | 0.344 |
Rpim | 0.022 | 0.177 |
Number of reflections | 27244 | 1966 |
<I/σ(I)> | 21.2 | 4.4 |
Completeness [%] | 99.8 | 99 |
Redundancy | 5.4 | 5.3 |
CC(1/2) | 0.999 | 0.909 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.16M calcium acetate, 0.08M sodium cacodylate pH6.5, 14.4% (w/v) PEG8000, 20% (v/v) glycerol |