6HRN
C-Phycocyanin from heterocyst forming filamentous cyanobacterium Nostoc sp. WR13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-07 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.91587 |
| Spacegroup name | P 63 |
| Unit cell lengths | 151.473, 151.473, 39.764 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 75.750 - 1.513 |
| R-factor | 0.11678 |
| Rwork | 0.115 |
| R-free | 0.15396 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | not published yet |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.612 |
| Data reduction software | XDS (20180808) |
| Data scaling software | pointless (1.11.14) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 131.179 | 1.589 |
| High resolution limit [Å] | 1.513 | 1.513 |
| Rmerge | 0.033 | 1.013 |
| Rmeas | 0.037 | 1.135 |
| Rpim | 0.016 | 0.470 |
| Number of reflections | 73328 | 3665 |
| <I/σ(I)> | 20.5 | 1.5 |
| Completeness [%] | 94.6 | 54 |
| Redundancy | 5.6 | 5.6 |
| CC(1/2) | 1.000 | 0.609 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | Morpheus crystallisation screen condition C4: 37.5% MPD_Peg1K_Peg3350 precipitant, 0.1M buffer system (imidazole & MES) and the NPS (nitrate, phosphate & sulfate) mix of additives. |
