6HR2
Crystal structure of PROTAC 2 in complex with the bromodomain of human SMARCA4 and pVHL:ElonginC:ElonginB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.332, 61.678, 81.603 |
| Unit cell angles | 69.43, 83.38, 86.42 |
Refinement procedure
| Resolution | 39.800 - 1.760 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.23600 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.940 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.800 | 1.960 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Number of reflections | 68052 | |
| <I/σ(I)> | 12.1 | |
| Completeness [%] | 89.4 | |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 20% (w/v) PEG1500, 0.1 M MIB Buffer, pH 6.0 |
