6HR0
Optimizing electroactive organisms: the effect of orthologous proteins
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-30 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97950 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 66.171, 66.171, 43.236 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.270 - 1.040 |
Rwork | 0.152 |
R-free | 0.17780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1m1p |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.270 | 1.070 |
High resolution limit [Å] | 1.040 | 1.040 |
Number of reflections | 44836 | 2412 |
<I/σ(I)> | 14.2 | |
Completeness [%] | 96.1 | |
Redundancy | 6.7 | |
CC(1/2) | 0.990 | 0.386 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1 M Bicine pH 8.5 and 3.7 M Ammonium Sulfate |