6HQJ
apo-form of polyphenol oxidase from Solanum lycopersicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-25 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9660 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.800, 54.140, 69.770 |
| Unit cell angles | 90.00, 101.08, 90.00 |
Refinement procedure
| Resolution | 42.468 - 1.802 |
| R-factor | 0.1655 |
| Rwork | 0.164 |
| R-free | 0.20060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6hqi |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.854 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.470 | 1.870 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rpim | 0.049 | 0.441 |
| Number of reflections | 40854 | |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 99.0 | |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 50 mM sodium citrate pH 6.6, 13% PEG 8000 |
