6HPQ
Crystal structure of human Pif1 helicase in complex with AMP-PNP, brominated crystal form.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-02 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.91966, 0.91992, 0.91912 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.250, 76.820, 142.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.370 - 1.430 |
| R-factor | 0.22579 |
| Rwork | 0.224 |
| R-free | 0.26633 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.958 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.250 | 1.470 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.146 | 2.316 |
| Number of reflections | 148682 | 10867 |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 12.8 | 10.7 |
| CC(1/2) | 0.999 | 0.598 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | Na-acetate, PEG 2K MME, Tris-Cl |
