6HPO
Crystallographic structure of the catalytic domain of Human Phenylalanine Hydroxylase (hPAH CD) in complex with iron at 1.6 Angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 65.856, 107.549, 124.016 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.770 - 1.670 |
| R-factor | 0.1615 |
| Rwork | 0.161 |
| R-free | 0.17700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4anp |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.773 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.770 | 1.730 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.084 | 1.921 |
| Rpim | 0.026 | 0.574 |
| Number of reflections | 51389 | 2603 |
| <I/σ(I)> | 16.2 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 40 mM PIPES, 20% PEG 2000, pH 6.8 |
