6HPF
Structure of Inactive E165Q mutant of fungal non-CBM carrying GH26 endo-b-mannanase from Yunnania penicillata in complex with alpha-62-61-di-galactosyl-mannotriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-18 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 98.988, 98.988, 170.497 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.240 - 1.360 |
| R-factor | 0.12304 |
| Rwork | 0.122 |
| R-free | 0.14345 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zm8 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.095 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.240 | 1.380 |
| High resolution limit [Å] | 1.360 | 1.360 |
| Number of reflections | 105928 | |
| <I/σ(I)> | 20.7 | |
| Completeness [%] | 100.0 | |
| Redundancy | 18.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Ammonium sulphate, Hepes pH 7.0. |
