6HPD
The structure of a beta-glucuronidase from glycoside hydrolase family 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-16 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.0332 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 157.929, 67.170, 96.720 |
Unit cell angles | 90.00, 95.93, 90.00 |
Refinement procedure
Resolution | 96.200 - 2.430 |
R-factor | 0.16704 |
Rwork | 0.165 |
R-free | 0.21149 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5dmy |
RMSD bond length | 0.008 |
RMSD bond angle | 1.162 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 96.200 | 2.520 |
High resolution limit [Å] | 2.430 | 2.430 |
Rmerge | 0.064 | 0.161 |
Number of reflections | 37999 | 3961 |
<I/σ(I)> | 11.3 | 5.8 |
Completeness [%] | 99.5 | 99.3 |
Redundancy | 10 | 9.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Sodium Bromide, 0.1M BIS-TRIS pH 7.5, 20% PEG 3350 |