6HPB

Crystal structure of the E.coli HicAB toxin-antitoxin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	293
Detector technology	PIXEL
Collection date	2017-12-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9794
Spacegroup name	P 32 2 1
Unit cell lengths	100.000, 100.000, 88.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	62.030 - 2.280
Rwork	0.241
R-free	0.27340
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AutoSol
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.030	2.362
High resolution limit [Å]	2.280	2.280
Rmerge	0.055
Number of reflections	23248	2320
<I/σ(I)>	1.5	1.5
Completeness [%]	97.5	100
Redundancy	2	2
CC(1/2)		0.672

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	0.1 M Sodium acetate 4.6 2 M Ammonium sulfate