6HOJ

Structure of Beclin1 LIR motif bound to GABARAP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	293
Detector technology	PIXEL
Collection date	2015-09-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97
Spacegroup name	P 21 21 2
Unit cell lengths	76.670, 150.490, 35.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.703 - 1.510
R-factor	0.1706
Rwork	0.169
R-free	0.21120
RMSD bond length	0.013
RMSD bond angle	1.221
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.703	1.560
High resolution limit [Å]	1.510	1.510
Rmerge	0.060	1.020
Rmeas	0.070	1.180
Rpim	0.030	0.570
Number of reflections	65391	6405
<I/σ(I)>	13.2	1.2
Completeness [%]	99.5	98.89
Redundancy	5.2	3.8
CC(1/2)	0.990	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	15% PEG 4000, 50 mM Bicine pH 8.8