6HNS

Structure of Nitrincola lacisaponensis flavin-containing monooxygenase (FMO) in complex with FAD and NADP+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-23
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000040
Spacegroup name	P 21 2 21
Unit cell lengths	59.057, 125.611, 144.725
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.480 - 1.840
R-factor	0.18325
Rwork	0.181
R-free	0.21724
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vq7
RMSD bond length	0.011
RMSD bond angle	1.616
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.480	1.870
High resolution limit [Å]	1.840	1.840
Rmerge	0.076	0.849
Number of reflections	93414	4431
<I/σ(I)>	14.7
Completeness [%]	99.2	97.1
Redundancy	4.4	4.4
CC(1/2)	0.999	0.585

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	8	277	0.2 Mg formate, 20% W/V PEG3350