6HM4
Crystal structure of Rad4 BRCT1,2 in complex with a Mdb1 phosphopeptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-10-11 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97950 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.170, 40.000, 54.960 |
Unit cell angles | 90.00, 105.16, 90.00 |
Refinement procedure
Resolution | 31.938 - 1.770 |
R-factor | 0.16857933455 |
Rwork | 0.166 |
R-free | 0.21141 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.833 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.940 | 1.833 |
High resolution limit [Å] | 1.770 | 1.770 |
Number of reflections | 19339 | |
<I/σ(I)> | 16.52 | |
Completeness [%] | 93.1 | |
Redundancy | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 287.15 | 200 mM Sodium acetate trihydrate, 100 mM Tris 8.5 and 30 % w/v PEG 4000 |